Dr. José M. Estevez
Dr. José M. Estevez is a Full Professor at UNAB (Chile) and Principal at the National Council for Scientific and Technical Research (CONICET) Argentina. He is a graduate of the Bachelor of Biological Sciences with an orientation in Botany from the Faculty of Natural Sciences of the University of La Plata (UNLP). In 2004 he obtained the title of Doctor in Biological Sciences from the Faculty of Exact Sciences at the University of Buenos Aires (UBA). In the period 2005-2008, he developed as a Post-Doctoral Fellow in the Laboratory of Dr. Chris Somerville at the Carnegie Institution at Stanford University, USA and at UC Berkeley, USA. From 2009 to May 2015 he directed the group of Molecular Bases of Plant Development at IFIBYNE (CONICET) and since June 2015 he has transferred his research group to the Leloir Institute Foundation (FIL)-IBBA. He is also currently the head of the laboratory at the UNAB Center for Plant Biotechnology (CBV).
Molecular Basis of Plant Development Lab
Molecular basis of cell size regulation in plant cells: using root hairs and pollen tubes of Arabidopsis thaliana as single cell models. Both pollen tubes and root hairs are single plant cells that play crucial roles in the survival of plants either in fertilization and seed formation or the acquisition of nutrients and water. Their cell expansion is polarized and directional. Our laboratory is studying the polarized cell expansion of both cell types in Arabidopsis thaliana as experimental model. Overall, we want to understand the molecular mechanisms that control in a highly regulated manner how plant cell expands. Both, root hairs and pollen tubes growth in a directional way integrating and sensing external signals (e.g. nutrients, microbiota) and also triggered by endogenous factors such as hormones (e.g. auxin). A better understanding of the impact of root hair cell development and the nutrient uptake as well as root anchoring in the soils when nutrients are one of the most important limiting factors to improve seeds and fruits productivity. In addition, pollen tubes growth plays crucial roles in the survival of plants either in fertilization and seed formation. This knowledge obtained will be applied to improve the nutrient uptake and water absorption as well as seeds production on important crops in Argentina like maize, wheat and soybean.
Research Line 1. Characterization of Cysteine-EndoPeptidases (CEPs) and apoplastic type-III peroxidases (PRXs) that impact on the assembly/disassembly of cell wall Extensins (EXT) glyconetwork and in the overall cell wall structure in growing root hairs. How CEP-PRXs are coordinated to manage the EXTs assembly and disassembly during polar growth?.
Research Line 2. Roles of RALF (Rapid Alkalinization Factor) peptides that control the signaling pathway involving Receptor Like Kinases FERONIA (FER) during root hair growth. How RALFs-FER-downstream components of the signaling cascades are linked to extracellular signals such nutrients/water and temperature?.
Research Line 3. Functions of Ca+2-ATPases ACAs and ECAs in root hair and pollen tube polar growth. How intracellular Ca2+ reservoirs are coordinated to contribute to the cytoplasmic Ca+2 gradient in these polar growing cells?.
Research Line 4. Identify the transcriptional network controlled by transcription factors activated at low-temperature in root hair cells using RNA-seq and CHIP-seq approaches. How temperature and nutrient deficient conditions as extracellular stimulus are decoded into a define transcriptional network in these single plant cells?.
Lic. Tomas Urzue. Research Asistant. [email protected]
Selected papers (2015-2020)
- Li L.1, Li B., Xie Ch., Zhang. T., Borassi C., Estevez JM*, Li X*#, Liu X* 2020. Arabidopsis RAD23B regulates pollen development by mediating KRP1 degradation. 2020. J. Exp. Bot. doi: 10.1093/jxb/eraa167. *Autor correspondiente.
- Borassi, C., Gloazzo Dorosz J., Ricardi, M. M. et al. J.M. Estevez*. 2020. A cell surface O-glycosylated peptide modulates root hair cell fate. New Phytologist. doi: 10.1111/nph.16487. *Autor correspondiente.
- Zhu S*, Estevez JM* et al. J. Martinez Pacheco, H. Guo, F. Yu. 2020. The RALF1–FERONIA complex phosphorylates eIF4E1 to promote localized protein synthesis and Polar Root Hair Growth. Mol Plant. doi.org/10.1016/j.molp.2019.12.014. *Co-Primer autor.
- 4. Mangano S., Denita-Juarez, S.P., Marzol, E., Borassi, C., and José M. Estevez. High auxin and high Pi impact on RSL2 and ROS-linked root hair growth in Arabidopsis. Frontiers in Plant Science. 9: 1164.doi: 10.3389/fpls.2018.01164. Autor correspondiente.
- 5. Sede, A.R, C. Borassi et al. José M. Estevez*and Jorge P. Muschietti*. 2018. Multiple Arabidopsis pollen extensin-like (PEXs) proteins are needed for cell wall integrity during pollen tube growth. FEBS Letters. doi:10.1002/1873-3468.12947. *Autor correspondiente.
- Mangano S., S.P. Denita Juarez, Choi, Marzol E. et al. & J.M. Estevez. 2017. Molecular link between auxin and ROS-controlled root hair growth. PNAS. 10.1073/pnas.1701536114. Autor correspondiente.
- 7. Mangano S., S.P. Denita Juárez & M. Estevez. 2016. ROS control of polar-growth in plant cells. Plant Physiology. 171(3): 1593-605. Autor correspondiente.
- Velasquez S.M., et al. & J.M. Estevez. 2015. Low sugar is not always goods: impact of specific O-glycan defects on tip-growth in Arabidopsis. Plant Physiology. 168(3): 808-13. Autor correspondiente.
- Velasquez S.M. et al. & Estevez J.M. 2015. Complex regulation of Prolyl-4-hydroxylase 5 impacts root hair expansion. Molecular Plant 8:734-746. Corresponding author.
Selected reviews (2015-2020)
- Zhu S, Martínez Pacheco J, Estevez JM*, & Yu F*. 2020. Autocrine regulation of root hair size by RALF-FERONIA-RSL4 signaling pathway. New Phytologist doi: 10.1111/nph.16497. *Corresponding author.
- Garcia Bossi J. et al. Estevez JM. 2020. Pumping Ca2+ out of the Cytoplasm: Functional Roles of Plant P-type IIA ECA and P-type IIB ACA Ca2+-ATPases in Arabidopsis thaliana. J. Exp. Bot. doi.org/10.1093/jxb/erz521.
- Mangano S.*, Martínez Pacheco, J.*, et al. J. M. Estevez. 2018. How does pH fit in oscillating polar growth?. Trends in Plant Sciences. 23(6):479-489. Corresponding author.
- Marzol, E.*, Borassi, C.*, et al. J. M. Estevez. 2018. Filling the gaps to solve the EXTENSIN puzzle. Molecular Plant. 11 (5): 645-658. doi: 10.1016/j.molp.2018.03.003. Corresponding author.
- Eliana Marzol et al. J.M. Estevez. 2017. RSL4 Takes Control: Multiple Signals; One Transcription Factor. Trends in Plant Science. 10.1016/j.tplants.2017.04.007. Corresponding author.
- Velasquez S.M., Jurgen Klein-Vein &J.M. Estevez. 2016. Auxin and cellular elongation. In Press. Plant Physiology. 170: 1206-1215. Corresponding author.
- Borassi C., Sede A.S. et al. Estevez J.M. 2016. An update on cell surface proteins containing Extensin-motif. J. Exp. Bot. 76(2): 477-487. Corresponding author.
National and International Collaborators
Cristophe Dunand. University of Toulouse (Touluse, France). Topic: «Plant PRXs involved in root hair growth». We have an strong colaboration on plant apoplastic PRXs since begining of 2016.
Jorge Muschietti. INGEBI (CONICET) (Buenos Aires, Argentina). Topic «P-type Ca2+-ATPases, ACAs and ECAs that regulate Ca2+ storage in organelles in polar growing cells: pollen tubes and root hairs».
Feng Yu. Hunan University (Changsha, China). Topic «RALFs-FER signalling pathway in root hair cells».
Marisa Otegui. University of Wisconsin-Madison (Madison-Wisconsin, US). Topic “TEM analysis of the plant cell wall structure in growing root hairs”.
Federico Ariel. Instituto de Agrobiotecnologia del Litoral (IAL), Santa Fe-Argentina. Topic “The long-non-coding RNA APOLO controls the expression of root hair specific genes”.
Award and Distinctions received
Accésit Award 2018 granted by the Argentine Society of Biology.
Houssay MINCyT Award 2016 Biological Sciences, Molecular Biology and Biochemistry area, awarded to researchers under 45 years of age.
Houssay 2014 SAB Award (Argentine Society of Biology): Special Mention.
National Academy of Sciences Award «Germán Burmeister 2013», Cell Biology and Genetics area.
Academy of Exact, Physical and Natural Sciences Award (ANCEFN) 2012 «Carlos Berg 2012», Biological Sciences area.
Finalist of the HHMI Early Career Competition 2011. November 2011.